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KMID : 1098420080160060409
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Korean Journal of Medicinal Crop Science
2008 Volume.16 No. 6 p.409 ~ p.415
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Moutan Cortex Extract Inhibits Amyloid β Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons
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Kim Ju-Yeon
Ju Hyun-Soo
Ban Joo-Yean
Song Kyung-Sik
Seong Yeon-Hee
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Abstract
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Moutan cortex, the root bark of Paeonia suffruticosa Andrews (Paeoniaceae), has pharmacological effects such as anti-inflammatory, antiallergic, analgesic and antioxidant activities. We investigated a methanol extract of Moutan cortex for neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25-35) in cultured rat cortical neurons. Exposure of cultured cortical neurons to 10 μM Aβ (25-35) for 24 h induced neuronal apoptotic death. Moutan cortex inhibited 10 μM Aβ (25-35)-induced neuronal cell death at 30 and 50㎍/㎖, which was measured by a 3 0[4,5dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Moutan cortex inhibited 10 μM Aβ(25-35)-induced elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) which were measured by fluorescent dyes. Moutan cortex also inhibited glutamate release into medium induced by 10 μM Aβ (25-35), which was measured by HPLC. These results suggest that Moutan cortex prevents Aβ (25-35)-induced neuronal cell damage by interfering with the increase of [Ca2+]i, and then inhibiting glutamate release and ROS generation. Moutan cortex may have a therapeutic role in preventing the progression of Alzheimer’s disease.
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KEYWORD
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Moutan Cortex, Amyloid β protein (25-35), Neurotoxicity, Alzheimer’s Disease, Neuroprotection
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